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Different parts of the C57 mouse brain, including the cortex, hippocampus, and cerebellum, are dissociated enzymatically and mechanically into suspension of single cells. Next, the cells are seeded, and the astrocytes are enriched in vitro,
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Image Search Results
Journal: CNS Neuroscience & Therapeutics
Article Title: RIPK3 activation promotes DAXX ‐dependent neuronal necroptosis after intracerebral hemorrhage in mice
doi: 10.1111/cns.14397
Figure Lengend Snippet: ICH promoted endogenous levels of RIPK3, AIF, DAXX, MLKL, and p‐MLKL in mice. (A) Representative western blot images. (B–D) Quantitative analyses of RIPK3, AIF, and DAXX at 12 h, 24 h, and 48 h in total proteins in the sham and ICH groups ( n = 3/group). (E) The relative level of p‐MLKL/MLKL at 12 h, 24 h, and 48 h in the sham and ICH groups ( n = 3/group). (F) Representative western blot images. (G–I) Quantitative analyses of RIPK3, AIF, and DAXX, at 12 h, 24 h, and 48 h in nuclear proteins in the sham and ICH groups ( n = 3/group). (J–O) Representative immunofluorescent images and quantification of RIPK3 (green), AIF (green), and DAXX (green) with NeuN (red, neuron marker) at 24 h in the sham and ICH groups ( n = 3/group). Scale bar = 50 μm. * p < 0.05 compared with sham.
Article Snippet: The following primary antibodies were mixed with cerebral slices at 4°C for an entire
Techniques: Western Blot, Marker